Progress in Optimization of Agrobacterium-Mediated Transformation in Sorghum (Sorghum bicolor)
نویسندگان
چکیده
منابع مشابه
Agrobacterium Mediated Transformation of Sorghum Bicolor for Disease Resistance
Sorghum serves as staple food for millions of people in Asia. It ranks as the sixth most planted crop and it is vulnerable to fungal diseases resulting in decreased grain quality and yield loss. Agrobacterium strains used were LBA4404 harbouring pcambia-ubi-chi11 (rice chitinase), EHA105 harbouring pcambia-ubi RC7 (rice chitinase) with bar gene and EHA105 harbouring pMKURF2 (rice chitinase gene...
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The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor ...
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A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase (PMI) gene, pmi, was used as the selectable marker gene and mannose was used a...
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Sorghum production is affected by a wide array of biotic constraints, of which sorghum shoot fly, Atherigona soccata is the most important pest, which severely damages the sorghum crop during the seedling stage. Host plant resistance is one of the major components to control sorghum shoot fly, A. soccata. To understand the nature of gene action for inheritance of shoot fly resistance, we evalua...
متن کاملGenetic engineering of grain sorghum (Sorghum bicolor (L
PhD thesis Sorghum (Sorghum bicolor L. Moench), the fifth most important cereal crop in the world, is one of the most important staple crops in Africa and South Asia and represents the only viable food grain for many of the world’s most food insecure people. However, sorghum grain shares the typical nutritional deficiencies of cereal grains, a low content of several essential amino acids. There...
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2018
ISSN: 1422-0067
DOI: 10.3390/ijms19102983